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Tropical Medicine Microscopy

Monica Cheesbrough

Published by Tropical Health Technology
2016, 2018, Updated 2022

Pages: 36     Colour plates: 137

This publication contains high quality colour plates of parasites, bacteria and fungi of medical importance in tropical countries, urine microscopy, blood cells and pathogens associated with HIV disease. The examination of samples and also staining techniques are described.

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CONTENTS
Use and care of a microscope
  • - Parasitic diseases: Malaria, African trypanosomiasis, Chagas' disease, leishmaniasis, lymphatic filariasis, loiasis, Mansonella infections, onchocerciasis, Cyclospora, Cystoisospora, Cryptosporidium, amoebiasis, giardiasis, soil transmitted helminths, intestinal schistosomiasis, foodborne trematodiases, balantidial dysentery, paragonimiasis, trichomoniasis, urogenital schistosomiasis..
  • - Features used to identify helminth eggs.
  • - Relative sizes of faecal parasites.
  • - Bacterial and fungal infections: Meningitis, staphylococci, streptococci, pneumococci, tuberculosis, sexually transmitted infections including gonorrhoea, syphilis, donovanosis, candidiasis, bacterial vaginosis. Campylobacter infection, cholera, leptospirosis, bartonellosis, plague, relapsing fever, leprosy, ringworm, toxoplasmosis, pneumococcal pneumonia, talaromycosis, Pneumoocystis pneumonia, histoplasmosis, aspergillosis.
  • - Pathogens associated with HIV infection.
  • - Urine microscopy: Pus cells and RBCs, spermatozoa, casts, crystals.
  • - Blood disorders: Normal RBC morphology, anaemia, sickle cell disease, haemoglobin C disease, thalassaemia, G6PD deficiency haemolysis, reticulocyte preparation, schistocytes, RBC inclusions, haemolytic disease of the newborn, basophilic stippling, spherocytes. Morphology of normal white blood cells, pyogenic bacterial infection, myelomatosis, reactive lymphocytes, leukaemia, Burkitt's. lymphoma.
  • - Techniques: Field staining, Giemsa staining, Leishman staining, Ziehl-Neelsen to detect Crytosporidium, Gram staining, Ziehl-Neelsen to detect AFB, Auramine-phenol staining to detect AFB, acridine orange staining, CSF cell count, examination of skin snips for Onchocerca volvulus microfilariae, examining CSF for trypanosomes, manual counting of WBCs, lyzed blood concentration to detect microfilariae, microscopical examination of urine, examination of faecal specimens.
  • Index